Journal: STAR Protocols

Article Title: Protocol for stimulating specific rodent limb receptive fields while recording in vivo somatosensory-evoked activity

doi: 10.1016/j.xpro.2024.102972

Figure Lengend Snippet: Schematic of the stimulation and recording set up (A) Different parts of the recording setup showing the acquisition system and the experimental table. Numbers represent: 1. stimulation unit; 2. stimulation unit battery; 3. digital stimulator; 4. Omniplex system; 5. DHP Amplifier; 6. Micromanipulator with electrode-headstage-adapter assemble; 7. stereotaxic frame; 8. ventilation mask for anesthetic influx; 9. lamp and surgical microscope; 10. homeothermic blanket. (B) Illustrative representation of the stimulation and recording setup. Numbers represent the same from panel A.

Article Snippet: In our case, we use a multichannel neural data acquisition system (Omniplex D System, Plexon Inc, USA) ( A-4) equipped with a digital input, an auxiliary analog input (up to 512 channels), a digital headstage processor (DHP) subsystem ( A-5) connected to the Omniplex D System chassis and auxiliary analog input ribbons that connect DHP to headstages ( A-6).

Techniques: Battery, Microscopy

Journal: STAR Protocols

Article Title: Protocol for stimulating specific rodent limb receptive fields while recording in vivo somatosensory-evoked activity

doi: 10.1016/j.xpro.2024.102972

Figure Lengend Snippet: Cranial window and ground electrode placement (A) Stereotaxic frame showing the animal on a homeothermic blanket held with ear bars on a ventilation mask. Note that aluminum foil is used to wrap the blanket to maintain animal temperature and decrease electrical noise. Numbers represent: 1. ventilation mask; 2. temperature sensor; 3. ear bars. (B) Expanded image of the animal head showing the ear bars and cranial sutures. Note that both lambda and bregma are easily observed after removing cranial skin. (C) Representation of bregma, lambda and the anatomical coordinate of the hindlimb somatosensory cortex on the right hemisphere (asterisk). (D) Visible cranial window (3 × 3 mm) within the white square. (E) View of the ground electrode (1 × 4 mm screw) placed posterior to lambda and the cranial window with the agar barrier. (F) General view of the electrode array and the ground cable. Numbers represent: 1. ground cable; 2. recording electrode assembled to the headstage. (G) Electrode array over the cortical surface of the right hindlimb cortex.

Article Snippet: In our case, we use a multichannel neural data acquisition system (Omniplex D System, Plexon Inc, USA) ( A-4) equipped with a digital input, an auxiliary analog input (up to 512 channels), a digital headstage processor (DHP) subsystem ( A-5) connected to the Omniplex D System chassis and auxiliary analog input ribbons that connect DHP to headstages ( A-6).

Techniques: